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By T. Haferlach, C. Schoch, W. Hiddemann (auth.), Prof. Dr. med. Wolfgang Hiddemann, Prof. Dr. Dr. med. phil. Torsten Haferlach, Dr. med. Michael Unterhalt, Prof. Dr. med. Thomas Büchner, Prof. Dr. med. Jörg Ritter (eds.)

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1995), Novel retroviral vectors for efficient expression of the multidrug resistance (mdr1) gene in early cells. J Virol. 69(12):7541-7 Hildinger M et al. (1998) FMEV vectors: both retroviral long terminal repeat and leader are important for high expression in transduced hematopoietic cells. Gene Therapy 5(11):1575-9 Marcucci G et al. (1998) Detection of minimal residual disease in patients with AMLl/ETO-associated acute myeloid leukemia using a novel quantitative reverse transcription polymerase chain reaction assay.

Except for cy-markers ("cy" for cytoplasmic versus "sm" for surface membrane) which were detected by immunostaining of cytospin slides, antigen positivity was examined by flow cytometry. rearrangement analysis, cytogenetic preparation and analysis, viral and mycoplasmal evaluation (Drexler, 2000; Drexler and Matsuo 1999; Drexler and Uphoff 2000; Drexler et al. 1997; Drexler et al. 1999; Drexler et al. 2001; Hu et al. 1996; Matsuo et al. 1997; Van Dongen and Wolvers-Tettero 1991). Results and Discussion A human leukemia cell line, named MUTZ-5, was established from the peripheral blood of a 26-year-old man with BCP-ALL at relapse in 1998.

Downing, J. , Selsted, M. , and Hiebert, S. W. (1998). The t( 8;21) fusion product, AML-l/ETO, associates with C/EBPa, inhibits C/EBPa-dependent transcription, and blocks granuloctyic differentiation, Mol Cell BioI 18, 322-333. Disturbed Differentiation of AMl1lETO Transduced Human (034+ Progenitors J. BASECKEt, CH. BAUM 2 , O. PFAHLERT l , G. BRITTINGERt, B. WORMANN3, and F. GRIESINGER l Introduction Material and Methods The translocation t( 8;21) (q22;q22), AMLl/ETO, one of the most frequent genetic aberrations in acute myelogenous leukemia is detectable in 40% of AML FAB M2.

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