By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the complex paintings of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – disguise a number of issues in pluripotent stem mobile culturing together with mouse and human fibroblasts, human embryonic stem cells and brought on pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in tradition is a finished choice of illustrated recommendations complemented by means of informative and academic captions analyzing what high quality cells seem like and the way they behave in a variety of environments. Examples of ideal cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and caused pluripotent stem phone colonies. This special and thorough atlas is a useful source for researchers, lecturers, and scholars who're attracted to or operating with stem mobilephone culturing.
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Additional info for Atlas of Human Pluripotent Stem Cells in Culture
Comparative study of mouse and human feeder cells for human embryonic stem cells. Int J Dev Biol. 2008;52:353–63. Fernandes AM, Meletti T, Guimarães R, Stelling MP, Marinho PA, Valladão AS, Rehen SK. Worldwide survey of published procedures to culture human embryonic stem cells. Cell Transplant. 2010;19:509–23. Lee JB, Song JM, Lee JE, Park JH, Kim SJ, Kang SM, et al. Available human feeder cells for the maintenance of human embryonic stem cells. Reproduction. 2004;128:727–35. Leonardo TR, Schell JP, Tran HT, Peterson SE.
Some human fibroblasts, when confluent, form swirl patterns. Overconfluent fibroblasts eventually become fragile and detach when left in cell culture. The cell morphology can change, and the cells can appear very long and thin. The cytoplasm can acquire a grainy appearance, and in some cases the cell membrane degrades. Fibroblasts are critical biological substrates for the maintenance of good-quality, undifferentiated PSCs. They form the basis on which robust, reliable, reproducible research will be founded, and it is well worth the time and effort to ensure that they are well sourced, free from contamination and appropriately frozen and banked, to guarantee their fitness for purpose.
A b c d Fig. 23 Images of old human feeders (a–c) compared with an optimal human feeder (d). a (Day 7 post-plating), b (Day 10) and c (Day 14) show a change over time in the morphology of the cells to an elongated, stretched, fragile cell morphology with evidence of cellular degeneration, unlike the optimal, robust fibroblast morphology seen in (d). Apoptotic cells are also evident in the old feeders (All images ×10 magnification) 34 Suggested Reading Amit M, Itskovitz-Eldor J. Morphology of human embryonic and induced pluripotent stem cell colonies cultured with feeders.