By Richard K. Burt
The original, compact, pocket-sized layout of Bone Marrow Transplantation makes this article a pragmatic. ready-reference for apartment officials, fellows, nurses and pharmacists at the bone marrow transplant unit. subject matters lined comprise: ailments requiring bone marrow transplants, reminiscent of a number of myeloma, lymphomas, pediatric malignancies and grownup strong tumors; symptoms, issues, drug doses and ways to scientific administration difficulties; unrelated donors, marrow processing, engraftment relapse and common sufferer care; and the necessities for preserving a bone marrow transplant unit.
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4. 1 Bone Marrow Transplantation 3) Leukocyte-Depleted Red Blood Cells Leukocytes express HLA antigens which may cause alloimmunization in the recipient, thereby increasing the risk of graft rejection or making it difficult to transfuse platelets, which also bear class I HLA antigens. Many centers, therefore, remove the leukocytes as a buffy coat at the time of initial centrifugation. Other centers go a step further and routinely use special leukocyte depletion filters for marrow recipients and patients who will need prolonged transfusion support, such as those with leukemia and aplastic anemia.
This is done to allow for collection of viable marrow cells, as povidoneiodine solution is cytotoxic to bone marrow cells. 4) If collecting samples for cytogenetic, flow cytometry or DNA analysis, rinse the syringes to be used for collection with approximately 1-2 ml of preservative-free heparin. No heparin is necessary for the syringe in which the initial sample is to be collected. 4% sodium bicarbonate buffer (in a 3:1 dilution), raise a skin weal over the crest. Anesthetize the deeper tissue with a 19 gauge needle being certain to reach the periosteum.
2. McGlave P, Bartsch G, Anasetti C et al. Unrelated donor transplantation therapy for CML; initial experience of the NMDP. Blood 1993; 81:543. 3. Gajewski J, Ho W, Feig S et al. Bone marrow transplantation using unrelated donors for patients with advanced leukemia or bone marrow failure. Transplant 1990; 50:244. 4. Beatty P, Hansen J, Longton G et al. Marrow transplantation from HLA-matched unrelated donor for treatment of hematologic malignancies. Transplant 1991; 51:443. 5. Stroncek D, Bartsch G, Perkins H et al.