By Lars Kaestner
The publication combines common suggestions and strategies to enquire calcium signalling in cells starting from molecular biology ways to manipulation of calcium in dwelling cells. the focal point inside those equipment in at the extensive diversity of fluorescence imaging know-how, particularly on optical sectioning concepts and quick photo acquisition. as well as those common directions there are software examples in a context past calcium signalling in significant fields: investigations of remoted cardiac myocytes and crimson blood telephone comparable examine. whereas the mobile cardiology part offers snapshots of yes calcium signalling points, the pink blood mobile half provides an outline from the practical id of calcium-channels to an idea of physiological and pathophysiological relevance.
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Additional info for Calcium signalling: Approaches and Findings in the Heart and Blood
In contrast to the pericard, the epicard is adhered to the myocard and can not be removed easily. Thus imaging of myocytes requires an extended light ⊡ Fig. 1 Non-linear imaging of human auricles. Panel A shows the macroscopic appearance of the atrial appendages; Aa – from outside the heart (epicardial side) and Ab from inside the heart, where the surgical cut was performed. The scale bar represents 5 mm. Panel B depicts laser scanning images taken from inside an atrial appendage as shown in image Ab.
Biochemical and biophysical research communications 72, 327–333 (1976). II Calcium Signalling Methodology Chapter 12 Non-linear and ultra high-speed imaging for explorations of the murine and human heart Lars Kaestner & Peter Lipp – 36 Chapter 13 A system for optical high resolution screening of electrical excitable cells – 45 Oliver Müller, Qinghai Tian, Roman Zantl, Valentin Kahl, Peter Lipp, Lars Kaestner Chapter 14 Concepts for optical high content screens of excitable primary isolated cells – 59 Lars Kaestner, Sandra Ruppenthal, Sarah Schwarz, Anke Scholz, Peter Lipp Chapter 15 A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventricular myocytes – 67 Cedric Viero, Udo Kraushaar, Sandra Ruppenthal, Lars Kaestner, Peter Lipp Chapter 16 Calcium imaging of individual erythrocytes: Problems and approaches – 83 Lars Kaestner, Wiebke Tabellion, Erwin Weiss, Ingolf Bernhardt, Peter Lipp 36 Chapter 12 · Non-linear and ultra high-speed imaging for explorations of the murine and human heart Non-linear and ultra high-speed imaging for explorations of the murine and human heart Lars Kaestner & Peter Lipp Reprint from Optics in Life Science, J.
3 · Results 12 inverted microscope (TE2000-U, Nikon, Japan) as previously described10. The illumination source was a monochromator (Polychrome IV, TILL Photonics GmbH, Germany). The spectra were spectrally corrected for the dichroic mirror and the emission filter. Transmission spectra of these optical components were measured using an absorption spectrometer (Genesys 6, Thermo Scientific, USA). 1 Non-linear imaging of human heart tissue - Second harmonic generation and autofluorescence imaging in living cardiac tissue For SHG imaging we employed a filter set that allowed simultaneous SHG and autofluorescence detection.